The ELISA kit employs a dual-antibody sandwich assay to accurately measure human thrombin levels in various biological specimens. The process begins with a microplate pre-coated with purified human thrombin-specific antibodies, creating a solid-phase antibody platform. Thrombin from the sample binds to these immobilized antibodies, followed by the addition of HRP-conjugated thrombin antibodies, forming a stable immune complex. After thorough washing to remove unbound components, the substrate TMB is introduced. Under the catalytic action of HRP, TMB turns blue and then shifts to yellow when an acidic stop solution is added. The intensity of the color developed is directly proportional to the concentration of thrombin present in the sample. A microplate reader is used to measure the absorbance at 450 nm, and the results are compared against a standard curve to determine the exact thrombin concentration.
Key features of this ELISA kit include:
1. High efficiency, sensitivity, and specificity due to the use of advanced antibodies.
2. Excellent reproducibility and reliability for consistent results.
3. A high-quality solid-phase carrier with strong adsorption capacity, low background, and clear optical properties.
4. Compatibility with multiple sample types, including serum, plasma, tissue homogenates, cell culture supernatants, and urine.
5. Cost-effective solution that helps reduce experimental expenses.
While this ELISA kit is widely used for monitoring and managing type 2 diabetes, it's important to note that current treatments primarily address the symptoms, such as hyperglycemia, rather than the root causes of the disease. Therefore, they offer control but not a cure.
Recent research highlights the critical role of the interaction between pancreatic beta cells and brain circuits, particularly the hypothalamus, in maintaining normal blood glucose levels. Scientists now believe that dysfunction in both the pancreatic and central nervous system mechanisms is a key factor in the development of type 2 diabetes. Understanding this partnership may lead to more effective therapeutic strategies in the future.
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