The ELISA kit utilizes a dual-antibody sandwich assay to accurately measure human thrombin levels in various biological specimens. The microplate is pre-coated with purified human thrombin-specific antibodies, forming a solid-phase antibody. Thrombin from the sample binds to these immobilized antibodies, followed by the addition of HRP-labeled secondary antibodies, creating an antibody-antigen-antibody complex. After thorough washing, the substrate TMB is added, which turns blue under the catalytic action of HRP and then changes to yellow upon acidification. The intensity of the color is directly proportional to the thrombin concentration in the sample. The absorbance is measured at 450 nm using a microplate reader, and the thrombin level is determined by comparing the results to a standard curve.
Key features of the kit include:
1. High efficiency, sensitivity, and specificity due to advanced antibody technology.
2. Excellent reproducibility and reliability for consistent results.
3. A high-quality solid-phase carrier with strong adsorption capacity, low background, and clear optical properties.
4. Suitable for a wide range of specimen types, including serum, plasma, tissue homogenates, cell culture supernatants, and urine.
5. Cost-effective solution that reduces overall experimental expenses.
While the ELISA kit is widely used to monitor and manage hyperglycemia in type 2 diabetes, it only addresses the symptoms rather than the root cause of the disease, making it a tool for control rather than cure. Recent studies suggest that normal glucose regulation relies on the collaboration between pancreatic beta cells and brain circuits, particularly those in the hypothalamus, which play a key role in maintaining blood sugar balance. Researchers now believe that dysfunction in both the pancreatic and central nervous system mechanisms is essential in the progression of type 2 diabetes, highlighting the need for more comprehensive therapeutic approaches.
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